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| Brand | MultiSciences |
| CatNum | 70-EK1101 |
| Product Name | Human sCD14 ELISA Kit |
| Customs Name | Human sCD14 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4 (unopened) standard stored at -20, others stored at 4 (opened) |
| Shipping Condition | 4 |
| Sample Volume | 100 l (prediluted) |
| Sensitivity | 2.58 pg/ml |
| Standard Curve Range | 125 - 8000 pg/ml |
| Spike Recovery Range | - |
| Mean Spike Recovery | - |
| CV of Intra plate | 3.2 % - 4.8 % |
| CV of Inter plate | 2.7 % - 4.6 % |
| Release Date | 2015/11/26 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against CD14 Human CD14 Standard, lyophilized CD14 Detect Antibody Streptavidin-HRP Assay Buffer (10) Substrate (TMB) Stop Solution Washing Buffer (20) Plate Covers |
| Describtion |
"This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CD14. The Human sCD14 ELISA is for research use only. Not for diagnostic or therapeutic procedures. CD14 is expressed mainly by macrophages and (at 10-times lesser extent) by neutrophils. It is also expressed by dendritic cells. It is a component of the innate immune system. CD14 acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). sCD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). sCD14 is secreted by the liver and monocytes and is sufficient in low concentrations to confer LPS-responsiveness to cells not expressing CD14. mCD14 and sCD14 are also present on enterocytes. sCD14 is also present in human milk, where it is believed to regulate microbial growth in the infant gut." |
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